论文部分内容阅读
目的:了解KAI1和COX-2在喉鳞癌组织中表达的情况,探讨两者在喉鳞癌组织中表达的意义及相关性,以及在喉鳞状细胞癌发生、发展及浸润转移过程中的作用。方法:应用免疫组织化学(SP)法分别检测45例喉鳞状细胞癌的咽喉黏膜组织和10名正常体检者咽喉黏膜组织中KAI1和COX-2的表达水平,分析两者的表达水平及强度与相关临床指标之间的关系,并分析这两个基因之间在喉鳞状细胞癌中表达的相关性。结果:KAI1基因在喉鳞癌细胞中的表达低于在正常咽喉黏膜组织中的表达,χ2=4.11,P=0.043;在喉癌组织中表达的阳性率与年龄、性别、分化程度、临床分期及是否有淋巴结转移均无关(P值均>0.05),但其阳性表达强度与临床分期及是否有淋巴结转移有显著相关性,P值均<0.05。COX-2基因在喉鳞癌组织中高表达,在正常咽喉黏膜组织中低表达,差异有统计学意义,χ2=15.36,P=0.000,在喉鳞癌组织中表达的阳性率与性别、年龄及分化程度无关(P值均>0.05),而与临床分期及是否有淋巴结转移有显著相关性,P值均<0.05。KAI1和COX-2在喉鳞癌组织中的表达呈负相关,r=-0.30,P=0.045。结论:KAI1和COX-2在喉鳞癌的发生、发展过程中具有一定抑制和促进作用,可作为今后预测肿瘤细胞转移潜能的指标。
OBJECTIVE: To investigate the expression of KAI1 and COX-2 in laryngeal squamous cell carcinoma (LSCC), and to explore the significance and correlation between the expression of KAI1 and COX-2 in laryngeal squamous cell carcinoma and their roles in the occurrence, development, invasion and metastasis of laryngeal squamous cell carcinoma effect. Methods: The expressions of KAI1 and COX-2 in the throat mucosa of 45 cases of laryngeal squamous cell carcinoma and 10 normal subjects were detected by immunohistochemistry (SP). The expression level and intensity And related clinical indicators, and analyzed the correlation between the two genes in laryngeal squamous cell carcinoma. Results: The expression of KAI1 in laryngeal squamous cell carcinoma was lower than that in normal laryngeal squamous cell carcinoma (χ2 = 4.11, P = 0.043). The positive expression rate of KAI1 in laryngeal squamous cell carcinoma was significantly correlated with age, sex, differentiation, clinical stage (P> 0.05). However, there was a significant correlation between the positive expression intensity and clinical stage and lymph node metastasis (P <0.05). COX-2 gene was highly expressed in laryngeal squamous cell carcinoma tissues and low in normal laryngeal mucosa tissues, the difference was statistically significant (χ2 = 15.36, P = 0.000). The positive expression rate of COX-2 in laryngeal squamous cell carcinoma was significantly related to sex, age, (P> 0.05). However, there was a significant correlation between clinical stage and lymph node metastasis (P <0.05). The expression of KAI1 and COX-2 in laryngeal squamous cell carcinoma was negatively correlated, r = -0.30, P = 0.045. Conclusions: KAI1 and COX-2 have some inhibitory and promoting roles in the occurrence and development of laryngeal squamous cell carcinoma, which can be used as an index to predict the metastatic potential of tumor cells in the future.