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目的检测早发型、晚发型重度子痫前期胎盘组织中骨转移蛋白-7(BMP-7)及其受体Ⅱ(BMPRⅡ)的表达情况,探讨其与重度子痫前期发病的关系。方法选取2014年9月-2016年1月在该院产科住院,临床确诊为早发型、晚发型重度子痫前期患者的胎盘组织作为研究对象,每组各30例;选取同期行择期剖宫产的正常相应孕周产妇的胎盘组织30例为对照组,分别采用qRt-PCR和Western Blot方法检测3组胎盘中BMP-7和BMPRⅡ中mRNA及蛋白的表达情况,免疫组化方法检测两者在胎盘中的表达定位。结果 BMP-7、BMPRⅡ的mRNA和蛋白表达水平在重度子痫前期胎盘组织中升高,且在晚发型重度子痫前期中升高更加明显,差异均有统计学意义(均P<0.05);BMP-7主要表达于胎盘绒毛合体滋养细胞层和绒毛外滋养细胞的细胞质,BMPRⅡ主要表达于胎盘绒毛滋养细胞和绒毛外滋养细胞的细胞膜。结论胎盘组织中BMP-7和BMPRⅡ的表达量增高有可能参与了重度子痫前期的发病过程。
Objective To detect the expression of bone morphogenetic protein-7 (BMP-7) and its receptor Ⅱ (BMPRⅡ) in early-onset and late-onset severe preeclampsia placenta and its relationship with severe preeclampsia. Methods From September 2014 to January 2016, the placenta tissues from hospitalized obstetrics and gynecology hospital, clinically diagnosed as early-onset and late-onset severe preeclampsia were selected as study subjects, with 30 cases in each group. Cesarean section The normal corresponding gestational age maternal placenta tissue 30 cases as the control group, qRt-PCR and Western Blot were used to detect the mRNA and protein expression of BMP-7 and BMPR Ⅱ in 3 groups of placenta, respectively, Placenta expression and localization. Results The mRNA and protein expression of BMP - 7 and BMPR Ⅱ increased in placenta of severe preeclampsia, and increased more significantly in late onset severe preeclampsia (all P <0.05). BMP-7 is mainly expressed in the cytoplasm of placental syncytiotrophoblast layer and extravillous trophoblast cells. BMPRⅡ is mainly expressed in the cell membrane of placental villous trophoblasts and extravillous trophoblast cells. Conclusion The increased expression of BMP-7 and BMPRⅡ in placenta may be involved in the pathogenesis of severe preeclampsia.