RNA干扰(RNAinterference,RNAi)是新近发展起来的一种静止基因表达的有效方法,是由双链RNA分子介导的特异性靶基因转录后基因沉默过程。1998年由Fire等〔1〕在对线虫的研究中首先提出,2001年Elbashir等〔2〕报道RNAi同样存在于哺乳动物细胞,这一突破性的进展使RNAi技术在哺乳动 RNAinterference (RNAi) is a newly developed effective method for the expression of quiescent genes. It is a process of gene silencing after specific target gene transcription mediated by double-stranded RNA molecules. In 1998 by Fire et al [1] first proposed in the study of nematodes, Elbashir et al [2] in 2001 reported that RNAi also exists in mammalian cells, this breakthrough advances RNAi technology in mammalian