Detection of canine echinococcosis by coproantigen ELISA

来源 :Asian Pacific Journal of Tropical Medicine | 被引量 : 0次 | 上传用户:qishi008
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Objective:To study the canine echinococcosis by coproantigen ELISA method.Methods:During the present investigation experimental infection was established using evaginated worms of Echinococcus granulosus(E.granulosus).To check cross reactivity two pups were infected with Taenia hydatigena(T.hydatigena).In order to detect the presence of antigen,hyperimmune sera were raised against excretory-secretory products of adult worms E.chinococcus granulosus. Faecal sample collected either from experimentally infected pups or from other sources were heated at 70℃to detect heat stable soluble antigen.Results:Pups harbouring less than 104 worms showed negative results.Samples collected from 14 days onwards from experimentally infected animals harbouring more than 104 worms showed positive value.The maximum positive samples were detected in samples collected from in and around slaughter house and the least number of samples were detected positive maintained by dog squad.Conclusions:The affinity purified IgG exhibited promising results for detection of canine echinococcosis by indirect ELISA. Objective: To study the canine echinococcosis by coproantigen ELISA method. Methods: established during the experimental investigation infection was established using evaginated worms of Echinococcus granulosus (E.granulosus). To check cross reactivity two pups were infected with Taenia hydatigena (T. hydatigena) .In order to detect the presence of antigen, hyperimmune sera were raised against excretory-secretory products of adult worms E.chinococcus granulosus. Faecal sample collected either from experimentally infected pups or from other sources were heated at 70 ° C to detect heat stable soluble antigen .Results: Pups harboring less than 104 worms showed negative results. Samples collected from 14 days onwards from experimentally infected animals more than 104 worms showed positive value. The maximum positive samples were detected in samples collected from in and around slaughter house and the least number of samples were detected positive maintained by dog ​​squad.Conclusions: The affinity p urified IgG A showing results for detection of canine echinococcosis by indirect ELISA.
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