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目的探讨雄激素缺乏对C57BL/6J雄鼠主动脉血管壁组织增龄性变化的影响。结论 24只8周龄C57BL/6J小鼠随机分为正常组(n=8)和去势组(n=8),另外8只C57BL/6J小鼠作为自然衰老组饲养18个月,测定各组血清睾酮浓度,并分离小鼠主动脉测定丙二醛(maleic dialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)的浓度,Western-blot测定去磷酸化Rb蛋白表达量以及提取主动脉线粒体DNA,用巢氏PCR技术分析线粒体DNA缺失突变率。结果与正常组相比,去势组血清睾酮浓度明显下降[(1.05±0.53)ng/mlvs(8.67±0.97)ng/ml,P<0.01];MDA浓度升高[(1.55±0.43)nmol/mgprotvs(2.42±0.41)nmol/mgprot,P<0.01];SOD浓度降低[(27.92±2.28)U/mlvs(17.09±1.71)U/ml,P<0.01],线粒体DNA缺失突变的突变率增加[(18.1713±2.4317)%vs(36.8475±3.3365)%,P=0.029];去磷酸化Rb蛋白表达量增加[(0.12±0.06)vs(0.36±0.07),P=0.001]。去势组与自然衰老组相比,以上衰老标志物差异均无统计学意义(P>0.05)。结论雄激素缺乏的小鼠主动脉组织衰老进程加快,内源性生理剂量的睾酮浓度能延缓雄性小鼠主动脉的衰老。
Objective To investigate the effect of androgen deficiency on the age-related changes of aortic vascular wall in C57BL / 6J male rats. Conclusions 24 C57BL / 6J mice aged 8 weeks were randomly divided into normal group (n = 8) and castration group (n = 8), and the other 8 C57BL / 6J mice were fed as natural aging group for 18 months. The concentration of serum testosterone was measured and the concentrations of maleic dialdehyde (MDA) and superoxide dismutase (SOD) were measured in the aorta of mice. The expression of dephosphorylated Rb protein and the content of superoxide dismutase Artery mitochondrial DNA, mitochondrial DNA deletion mutation rate was analyzed by nested PCR. Results Compared with the normal control group, the serum testosterone concentration was significantly decreased in the castration group [(1.05 ± 0.53) ng / ml vs (8.67 ± 0.97) ng / ml, P <0.01] (27.92 ± 2.28) U / ml vs (17.09 ± 1.71) U / ml, P <0.01], and the mutation rate of mitochondrial DNA deletion mutation increased (P <0.01) (18.1713 ± 2.4317)% vs (36.8475 ± 3.3365)%, P = 0.029]. The level of dephosphorylated Rb protein increased ([0.12 ± 0.06] vs (0.36 ± 0.07), P = 0.001]. There were no significant differences in the above-mentioned senescence markers between castration group and natural aging group (P> 0.05). Conclusion The aging process of the aorta in androgen-deficient mice is accelerated, and the endogenous physiological dose of testosterone can delay the aging of the aorta in male mice.