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肺癌出现各种表现,导致组织学分类复杂。分类的目的是指导临床治疗和估价预后。目前根据光镜所见常分为鳞癌、腺癌、小细胞癌和未分化癌(巨细胞癌)4类。电镜、免疫细胞化学和流体细胞计数技术的应用,对肺癌的这种分类提出了某些疑问。本文对66例有原发性肺癌的肺标本同时进行了光镜、电镜和免疫细胞化学的研究。手术取出的肺癌标本立即分取3块组织分别进行下述3种处理:4%甲醛盐水处理,在光镜下观察结果;组织立即用液氮骤冻,进行免疫细胞化学研
Various manifestations of lung cancer have led to complex histological classification. The purpose of classification is to guide clinical treatment and evaluate prognosis. Currently classified according to light microscopy, it is often classified into squamous cell carcinoma, adenocarcinoma, small cell carcinoma, and undifferentiated carcinoma (macrocytic carcinoma). The use of electron microscopy, immunocytochemistry, and fluid cell counting techniques raises some questions about this classification of lung cancer. In this paper, light microscopy, electron microscopy, and immunocytochemistry were performed on 66 lung specimens with primary lung cancer. The surgically extracted lung cancer specimens were immediately divided into 3 groups and subjected to the following three treatments: 4% formalin solution, observed under light microscopy, and immediately frozen in liquid nitrogen for immunocytochemistry.