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目的研究血管紧张素原(AGT)基因M235T及血管紧张素Ⅱ1型受体(ATIR)基因A1166/C多态性与冠心病的关系。方法应用多聚酶链反应结合限制性内切酶法(PCR-RFLP)对100名经冠脉造影确诊为冠心病(CHD)的患者和52名冠造结果正常及18名门诊常规体检且无冠心病史者基因多态性进行分析。结果冠心病(CHD)组TT基因型和T等位基因频率显著高于对照组(分别为0.67、0.79和0.44、0.63,P<0.05),而C等位基因频率在两组中差异则无统计学意义(分别为0.075和0.064,P>0.05)。结论AGT基因变异与CHD发病具有相关性。提示T等位基因可能是CHD的危险因素,而AT1R基因A1166/C多态性与冠心病则无关联。
Objective To investigate the relationship between angiotensinogen (AGT) gene M235T and angiotensin Ⅱ type 1 receptor (ATIR) gene A1166 / C polymorphism and coronary heart disease. Methods 100 patients with coronary heart disease (CHD) diagnosed by coronary angiography and 52 patients with normal coronary arteries and 18 normal subjects with out-patient clinic without coronary heart disease were analyzed by polymerase chain reaction and restriction endonuclease assay (PCR-RFLP) History of genetic polymorphisms were analyzed. Results The frequencies of TT genotype and T allele in CHD group were significantly higher than those in control group (0.67,0.79 and 0.44,0.63, respectively, P <0.05), while the frequency of C allele was not significantly different between the two groups Statistical significance (0.075 and 0.064 respectively, P> 0.05). Conclusion There is a correlation between AGT gene mutation and CHD incidence. T allele may be a risk factor for CHD, while AT1R gene A1166 / C polymorphism and coronary heart disease is not associated.