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目的:观察银杏叶提取物(GbE)对糖尿病大鼠模型膈肌葡萄糖摄取率和葡萄糖转运体4(GLUT4)mRNA表达水平的影响。方法:40只雄性SD大鼠随机分为正常对照组10只,造模组30只。应用高糖高脂饮食加小剂量链脲佐菌素诱发糖尿病大鼠模型。随机选取造模成功大鼠20只均分成2组:糖尿病组、GbE治疗组。GbE治疗组按8mg·kg-1.d-1剂量腹腔注射GbE,持续8周。检测各组大鼠空腹血糖和胰岛素水平,膈肌组织葡萄糖摄取率和GLUT4 mRNA水平,并观察其超微结构的变化。结果:与正常对照组比较,糖尿病组大鼠空腹血糖和胰岛素升高(P<0.01);膈肌葡萄糖摄取率和GLUT4 mR-NA水平下降(P<0.05,P<0.01);电镜下主要表现为膈肌线粒体扩张,嵴变短,空泡化。GbE治疗组上述变化明显减轻。结论:GbE可部分纠正糖尿病大鼠模型高血糖、高胰岛素状态,并减轻膈肌损伤,其作用与增强膈肌GLUT4基因表达,促进膈肌对葡萄糖的摄取和利用有关。
Objective: To observe the effects of Ginkgo biloba extract (GbE) on glucose uptake and expression of glucose transporter 4 (GLUT4) mRNA in diaphragmatic muscle of diabetic rats. Methods: Forty male SD rats were randomly divided into normal control group (n = 10) and model group (n = 30). Application of high-sugar, high-fat diet plus small doses of streptozotocin induced diabetic rat model. Twenty randomly selected rats were divided into two groups: diabetic group and GbE-treated group. The GbE group was injected intraperitoneally with GbE at 8 mg · kg-1.d-1 for 8 weeks. Fasting blood glucose and insulin levels, glucose uptake rate and GLUT4 mRNA level in diaphragmatic muscle tissue were detected in all groups, and their ultrastructural changes were observed. Results: Compared with normal control group, fasting blood glucose and insulin increased (P <0.01) in diabetic rats, glucose uptake rate and GLUT4 mR-NA decreased (P <0.05, P <0.01) Diaphragm mitochondria dilated, short crest, vacuolar. GbE treatment group significantly reduced the above changes. Conclusion: GbE can partially correct hyperglycemia and hyperinsulinemia in diabetic rats and alleviate diaphragmatic injury, which is related to the enhancement of GLUT4 gene expression in diaphragmatic muscle and the promotion of diaphragmatic glucose uptake and utilization.