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为明确甘薯色素、干率与肉色的关系,给图像RGB特征值评估甘薯色素含量提供依据,测定了甘薯240份品系的胡萝卜素含量及234份品系的花青素含量、干率和薯肉RGB颜色特征值。结果表明,甘薯胡萝卜素含量与RGB值的相关系数r=-0.913,偏相关系数为-0.872,花青素含量与RGB值的相关系数r=-0.838,偏相关系数为-0.836,说明甘薯色素含量与肉色是高度正相关的,是肉色的决定因子,但胡萝卜素与肉色关系更紧密。干率与胡萝卜素RGB值的相关系数r=0.597,偏相关系数为0.273,干率与花青素RGB值的相关系数r=-0.273,偏相关系数为0.255,说明干率与对肉色有极明显的负面影响,但是在RGB值≥200的低胡萝卜素(约含量3 mg/100 g·FW以下)群体中,干率与RGB值的偏相关系数为-0.0066(P=0.9426),干率对低胡萝卜素含量品系的肉色没有影响。采用R值、G值、B值、干率的二次多项式回归模型,RGB值≥200的低胡萝卜素群体、RGB值<200的较高或高胡萝卜素群体和花青素群体的拟合方程的复相关系数R分别为0.975、0.982和0.937,说明拟合值与实测值关系十分密切。对胡萝卜素含量高于3 mg/100 g·FW的品种,拟合值能达到约90%的精确度,对胡萝卜素含量低于3 mg/100g·FW的品种,也能达到约83%的精确度,对花青素品种能达到约86%的精确度。因此,图像RGB特征值法不仅能够很好地反映甘薯品种间的色素差异,还能较精确地估测甘薯色素含量,可为甘薯早代育种的大批量样品提供便捷、客观的色素含量评估方法。
In order to clarify the relationship between sweetpotato pigment, dry rate and flesh color, the pigment content of sweetpotato was evaluated based on the RGB characteristic value of the image. The carotenoid content of 240 sweetpotato lines and the anthocyanin content of 234 lines, Color characteristic value The results showed that the correlation coefficient of carotene content with RGB value was -0.913, the partial correlation coefficient was -0.872, the correlation coefficient of anthocyanin content with RGB value was -0.838, partial correlation coefficient was -0.836, which indicated that the content of sweet potato pigment Content and flesh color is highly positive, is the decisive factor of flesh color, but carotene and flesh color more closely. The correlation coefficient between dry rate and carotene RGB value was 0.597 and the partial correlation coefficient was 0.273. The correlation coefficient between dry rate and anthocyanin RGB value was -0.273, partial correlation coefficient was 0.255, (P = 0.9426). The partial correlation coefficient of dry rate to RGB value was -0.0066 (P = 0.9426) in the population of low-carotene with the RGB value ≥200 (about 3 mg / 100 g · FW) No effect on flesh color of low-carotene content lines. The quadratic polynomial regression model with R value, G value, B value, and dry rate, the low carotene population with RGB values ≥200, the fitting equation of higher or high carotene population with anthocyanin group with RGB value <200, The complex correlation coefficient R were 0.975,0.982 and 0.937, indicating that the fitting value and the measured value is very close. For the cultivars with more than 3 mg / 100 g · FW of carotene, the fitted value can reach about 90% accuracy, and about 83% of the cultivars with carotene content less than 3 mg / 100 g · FW Accuracy, about 86% accuracy for anthocyanin varieties. Therefore, the image RGB eigenvalue method not only can reflect the difference of pigment between sweet potato varieties well, but also can estimate the content of sweet potato pigment more accurately, which can provide a convenient and objective evaluation method of pigment content for the early batch breeding of sweet potato .