论文部分内容阅读
目的 观察多沙唑嗪对激素非依赖性前列腺癌DU- 145细胞增殖与凋亡的影响并探讨其作用机理。 方法 DU -145细胞培养液中加入多沙唑嗪,使其终浓度分别为0. 1、1、10、25、100μmol/L,不加多沙唑嗪者为对照组。24、48、72、96h后分别检测各组癌细胞的吸光度A值,并计算细胞生长抑制率,取与对照组比较有显著性差异的最低浓度为多沙唑嗪最佳浓度,取有显著性差异的最短时间为最佳作用时间。分析25μmol/L多沙唑嗪作用48h后的细胞周期分布及早期凋亡率,观察细胞形态,检测凋亡相关因子及蛋白的表达。 结果 0. 1、1、10μmol/L组的A值与对照组相比差异无统计学意义; 25、100μmol/L组的A值与对照组相比差异有统计学意义(P<0. 01); 25μmol/L多沙唑嗪作用48h时即出现统计学差异。25μmol/L多沙唑嗪作用48h后,细胞周期分布情况与对照组相比差异无统计学意义;早期凋亡率为14. 31%,与对照组(1. 07% )相比差异有统计学意义;电镜观察显示部分细胞呈现典型的凋亡形态学改变;细胞转化生长因子β1 (TGF- β1 )呈阳性表达,Fas表达增强,bcl -2表达与对照组比较差异无统计学意义。 结论 多沙唑嗪可能通过Fas系统或直接激活TGF -β1 信号通路介导凋亡的发生,从而抑制激素非依赖性前列腺癌细胞生长。
Objective To observe the effect of doxazosin on the proliferation and apoptosis of hormone-independent prostate cancer DU-145 cells and to explore its mechanism. Methods Doxazosin was added into DU-145 cell culture medium to make the final concentrations of 0. 1, 10, 25 and 100 μmol / L, respectively. 24, 48, 72, 96h after the detection of each group of cancer cells absorbance A value, and calculate the cell growth inhibition rate, compared with the control group was the lowest concentration of the best concentration of doxazosin, whichever is significant The shortest time of sexual difference is the best time for action. The cell cycle distribution and early apoptosis rate after 25μmol / L doxazosin treatment for 48h were observed. The cell morphology was observed and the apoptosis related factors and protein expression were detected. Results 0.1,1μmol / L group A value compared with the control group was no significant difference; 25,100μmol / L group A value compared with the control group, the difference was statistically significant (P <0.01 ); 25μmol / L doxazosin 48h when there is a statistical difference. The effect of 25μmol / L doxazosin on the cell cycle distribution was not significantly different from that of the control group at 48h (P <0.05). The early apoptosis rate was 14.31%, which was statistically different from the control group (1. 07%) There was no significant difference in the expression of bcl-2 protein between the control group and the control group (P> 0.05). The changes of apoptosis in some cells were observed by electron microscope. The expression of TGF-β1 was positive. CONCLUSION Doxazosin can inhibit the growth of hormone-independent prostate cancer cells through the Fas system or directly through the activation of TGF-β1 signaling pathway.