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为了解参与机体免疫调控过程的中枢神经核团，对雄性ＳＤ大鼠侧脑室及腹腔注射多克隆免疫激发剂ＬＰＳ，应用即刻早期表达基因ｃ－ｆｏｓ免疫组织化学法，观察大鼠脑内Ｆｏｓ表达。侧脑室注射ＬＰＳ１０ｎｇ，３ｈ后见下丘脑室旁核内侧部对应ＣＲＦ神经元分布区有密集的Ｆｏｓ阳性染色；外侧及腹侧部相当于ＯＸＴ细胞所在部位的脑干、脊髓投射区有Ｆｏｓ表达细胞；前大细胞亚核亦有Ｆｏｓ染色细胞。视上核、杏仁中央核也观察到较为密集的Ｆｏｓ阳性细胞。腹腔注射ＬＰＳ１００μｇ后０．５ｈ，于侧脑室注射表达Ｆｏｓ的部位见有阳性着色细胞，３ｈ达高峰，随后逐渐降至对照组水平。腹腔注射ＬＰＳ剂量反应曲线显示，５．０μｇ即可引起Ｆｏｓ表达，１００μｇＦｏｓ表达细胞数达高峰。腹腔注射１００μｇ，３ｈ后，下丘脑内Ｆｏｓ表达与侧脑室注射者相似。且在孤束核有密集的Ｆｏｓ表达细胞，经ＴＨ抗体复染，４０％的Ｆｏｓ阳性细胞被双标。结果表明：ＬＰＳ可激活脑内多个神经核团，提示大脑对免疫功能的调控是多部位的协调作用。孤束核参与免疫激发信息的上传；下丘脑室旁核在大脑调控信息下传至免疫器官中起重要作用，其不同亚核分别向垂体前叶、后叶、脑干及脊髓的投射，似可组成３条通路：即下丘?
In order to understand the central nervous nuclei involved in the process of immune regulation, the polyclonal immunostimulant LPS was injected into lateral ventricle and peritoneal cavity of male SD rats to detect the expression of Fos in rat brain by c-fos immunohistochemistry . Intracerebroventricular injection of LPS10ng, 3h after the hypothalamic paraventricular nucleus medial corresponding CRF neuronal distribution dense Fos-positive staining; lateral and ventral part of the brain stem cells where the location of OXT, spinal cord projection area with Fos cells ; Pre-large sub-nuclear cells also Fos staining. As the supranuclear, alveolar central nucleus also observed more dense Fos-positive cells. After intraperitoneal injection of 100μg of LPS for 0.5h, positive staining cells were observed in the site of Fos injected into the lateral ventricle and peaked at 3h, then gradually decreased to the level of the control group. The intraperitoneal injection of LPS dose response curve showed that 5.0μg can cause Fos expression, 100μgFos expression of cells reached the peak. Intraperitoneal injection of 100μg, 3h, Fos expression in the hypothalamus and lateral ventricle were similar. And in the nucleus tractus solitarius dense Fos-expressing cells, stained with TH antibody, 40% of Fos-positive cells were double-labeled. The results showed that: LPS can activate multiple brain nuclei, suggesting that the regulation of immune function of the brain is a multi-part coordination. Nucleus tractus solitarius participates in the uploading of immune-stimulated information; hypothalamic paraventricular nucleus plays an important role in the regulation of brain regulatory information to the immune organs. The different sub-nuclei of the hypothalamus play an important role in the anterior pituitary gland, posterior lobe, brain stem and spinal cord, Can be composed of three access roads: the next hill?