论文部分内容阅读
AIM:To investigate the effect of flavone on oxidation-induced injury in retinal pigment epithelium cells. METHODS:In in vivo studies,NaIO_3-induced RPE degeneration in rat eyes were treated with 0.5%flavone eye drops 3 times a day for 1 week before and 4 weeks after NaIO_3 injection.At the end of 2 and 4 weeks,all rats were measured c-wave by electroretinogram(ERG).In in vitro studies,ARPE-19 cells were treated with hypoxia,H_2O_2 NaN_3 and t-BHP to induce cell damages.MTT assay was used to measure the viable cells. ? RESULTS:The ERG c-wave results showed that flavone reversed NaI03-induced injury at the end of 4 weeks.In v/tro results showed flavone reversed the various oxidants-induced injuries in RPE cells. ? CONCLUSION:Flavone could prevent the RPE from oxidation- induced injury both /n wVoar6 /n w’tro.
A: To investigate the effect of flavone on oxidation-induced injury in retinal pigment epithelium cells. METHODS: In in vivo studies, NaIO_3-induced RPE degeneration in rat eyes were treated with 0.5% flavone eye drops 3 times a day for 1 week before and 4 weeks after NaIO_3 injection. At the end of 2 and 4 weeks, all rats were measured c-wave by electroretinogram (ERG). In vitro studies, ARPE-19 cells were treated with hypoxia, H_2O_2 NaN_3 and t-BHP to induce cell damage. MTT assay was used to measure the viable cells.? RESULTS: The ERG c-wave results showed that induced injuries in RPE cells.? CONCLUSION: Flavone could prevent the RPE from oxidation-induced injury both / n wVoar 6 / n w’tro.