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目的：比较美洲钩虫及十二指肠钩虫线粒体细胞色素Ｃ氧化酶１（ＣＯ１）基因序列，确定两种钩虫间ＣＯ１基因差异。方法：现场采集钩虫成虫样本，蛋白酶Ｋ消化抽提线粒体基因组ＤＮＡ，ＰＣＲ方法扩增ＣＯ１基因，ＤＮＡ测序及分析。结果：ＰＣＲ扩增获得约７００ｂｐ美洲钩虫及十二指肠钩虫ＣＯ１基因片段，其序列比较显示两种钩虫ＣＯ１基因同源性达８９．７％，存在特定的核苷酸差异。结论：ＣＯ１基因可作为鉴别两种人体钩虫虫种标记性基因。
OBJECTIVE: To compare the mitochondrial cytochrome C oxidase 1 (CO1) gene sequences between hookworm and hookworm of hookworm, and determine the difference of CO1 gene between two hookworm. Methods: Adult samples of hookworm were collected on the spot, mitochondrial genomic DNA was digested with protease K, CO1 gene was amplified by PCR, and DNA sequencing and analysis were performed. Results: The CO1 gene fragment of Hookwort and Hookworm of about 700bp was amplified by PCR. The sequence comparison showed that the homology of CO1 gene of the two hookworm was 89.7%, and there were specific nucleotide differences. Conclusion: The CO1 gene can be used as a marker for the identification of two species of human hookworm.