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长角血蜱在吸血时 ,其唾液腺中的 apyrase可明显地抑制由 A DP所诱导的血小板聚集反应 ,并呈剂量反应关系 ,1/ 2对唾液腺可以完全抑制血小板的聚集反应。通过 apyrase的动力学、 HPLC分子筛、等电聚焦电泳和温度敏感性实验证明 ,水解 ATP和 ADP并不是 ATP酶、 ADP酶或几种酶共同作用的结果 ,而是一种酶即 apyrase的作用结果。Apyrase对 Ca2 +具有依赖性。而 Mg2 +、Zn2 +、Mn2 +对之影响较小 ,Hg2 +和EDTA是 apyrase的抑制剂。吸血对蜱唾液腺 apyrase活性影响较大 ,吸血 6天时其活性最大 ,吸血后活力明显下降。A pyrase与哺乳动物的 5′-核苷酸酶相似 ,它是一种糖基 -磷脂酰肌醇 ( GPI)膜锚结构蛋白。
In the process of blood-sucking, H. japonicus apyrase in salivary gland could significantly inhibit the platelet aggregation induced by A DP, and showed a dose-response relationship. One-half of the salivary glands could completely inhibit platelet aggregation. By apyrase kinetics, HPLC molecular sieve, isoelectric focusing electrophoresis and temperature-sensitivity experiments show that the hydrolysis of ATP and ADP is not the result of ATPase, ADP enzyme or several enzymes together, but the result of an enzyme that apyrase . Apyrase is Ca2 + dependent. However, Mg2 +, Zn2 + and Mn2 + had little effect on them, and Hg2 + and EDTA were inhibitors of apyrase. Vampire had a significant effect on the activity of apyrase in tick salivary glands, and its activity was the highest at 6 days after vampire injection. A pyrase, like mammalian 5’-nucleotidase, is a glycosyl-phosphatidylinositol (GPI) membrane anchor protein.