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目的 :为研究油烟凝聚物与肿瘤发生的潜在关系及大蒜油的防护作用。方法 :用油烟凝聚物和大蒜油分别或同时处理V79细胞 ,利用免疫细胞化学方法检测V79细胞中 p5 3、p2 1ras蛋白的表达情况。结果 :与对照组相比 ,油烟凝聚物处理组 p5 3、p2 1ras蛋白表达明显升高 (P <0 .0 1) ,且有剂量———效应关系 ;与对照组相比 ,5 0 μl/ml大蒜油处理组降低油烟凝聚物诱发V79细胞 p5 3、p2 1ras蛋白表达水平 (P <0 .0 5或P <0 .0 1)。结论 :油烟凝聚物有诱变作用 ,大蒜油可防护油烟凝聚物的诱变作用。
Objective: To study the potential relationship between coke agglutinant and carcinogenesis and the protective effect of garlic oil. Methods: V79 cells were treated with oil fume condensate and garlic oil respectively, and the expression of p5 3 and p2 1ras in V79 cells was detected by immunocytochemistry. Results: Compared with the control group, the expressions of p5 3 and p2 1ras proteins were significantly increased in the fucoidan-treated group (P <0.01), and there was a dose-effect relationship. Compared with the control group, / ml garlic oil treatment group decreased the expressions of p5 3 and p2 1ras protein in V79 cells induced by fucoidan (P <0.05 or P <0.01). Conclusion: The fume condensate has the mutagenic effect. Garlic oil can protect the mutagenesis of fume condensate.