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本工作用紫外分光光度法、磷比色法以及电子自旋共振研究发现临床应用有显效的三种保护剂——半胱氨酸,对甲苯磺酸盐,S-(2-氨乙基)异硫脲氢溴酸盐(AET)和2,2-二甲基四氢噻唑盐酸盐(DMTD)对胸腺嘧啶和脫氧胸腺嘧啶核苷酸具有明显的保护效应。通过巯基浓度的测定与ESR波譜特征的鉴别,协同証实AET的保护效应不仅是由于它在水溶液中轉化为巯基乙胍(MEC)所致,AET分子中的=N-H基团也是呈現保护作用的重要功能团,而半胱氨酸对甲苯磺酸盐和DMTD的保护作用无疑地应归结于这些化合物结构中所含或水解释放的巯基基团。
This work using UV spectrophotometry, phosphorus colorimetric and electron spin resonance studies found that the clinical application of effective three protective agents - cysteine, p-toluenesulfonate, S- (2-aminoethyl) Isothiourea hydrobromide (AET) and 2,2-dimethyl tetrahydrothiazole hydrochloride (DMTD) have a significant protective effect on thymine and deoxythymidine. Through the determination of the sulfhydryl concentration and the identification of the ESR spectral features, the synergistic effect of the protective effect of AET was confirmed not only by its conversion to mercaptoethanol (MEC) in aqueous solution, but also by the = NH group in the AET molecule Important functional groups, while the protective effect of cysteine p-toluenesulfonate and DMTD are undoubtedly due to the thiol groups contained in the structure of these compounds or hydrolyzed.