论文部分内容阅读
业已发现,完整牛乳头瘤病毒1型(BPV1)的超免疫血清与牛乳头瘤病毒2型(BPV2)之间具有强烈交叉反应,乳头瘤病毒(PV)具有属特异性和型特异性抗原。本文报道用BPV1单克隆抗体进行研究的结果。给6周龄雌性BALB/c小鼠接种SDS裂解的BPV1,取脾细胞与小鼠骨髓瘤细胞系P3×63Ag8U1融合。用ELISA筛选与SDS裂解BPV1结构蛋白发生反应的免疫球蛋白分
It has been found that there is a strong cross-reactivity between the fully immunized sera of bovine papillomavirus type 1 (BPV1) and bovine papillomavirus type 2 (BPV2), which are both genus-specific and type-specific antigens. This article reports the results of a study using BPV1 monoclonal antibodies. Six-week-old female BALB / c mice were inoculated with SDS-lysed BPV1 and the spleen cells were fused with the mouse myeloma cell line P3 × 63Ag8U1. Immuno-globulin fractions that reacted with the SDS-cleaved BPV1 structural protein were screened by ELISA