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采用傅里叶变换红外(FTIR)光谱法并结合二维相关(Two-dimensional correlation spectroscopy)分析技术分别对药用植物甘草(药材对照品)及其伪品刺果甘草进行了无损快速鉴别研究。结果表明:虽然甘草和伪品刺果甘草都是来源于同一科属,但两者所含化学组分的含量不同,其红外光谱图既有一定的差异,又有一定的相似。而在红外二阶导数谱图上差异较明显,在二维红外谱图的差别不但较明显而且很直观。凭借这些差异,可方便地进行真伪鉴别,同时还进一步表明了这两种甘草药材中化学组分之间的差异。该法无损,快速,准确,样品量少,为客观评价中药材的来源,真伪,优劣等提供了一种新的方法和手段。
The non-destructive and rapid identification of medicinal plant licorice (medicinal reference substance) and its counterfeit gingo licorice root were carried out by Fourier transform infrared (FTIR) spectroscopy and two-dimensional correlation spectroscopy. The results showed that although both licorice root and licorice rooted in the same genus, the contents of their chemical components were different. The infrared spectra of the two species were not only different but also similar. In the second derivative infrared spectrum difference is more obvious difference in the two-dimensional infrared spectrum is not only more obvious and very intuitive. With these differences, authenticity can be easily identified and the differences between the chemical components of the two licorice herbs further demonstrated. The method is nondestructive, rapid, accurate and has a small amount of samples. It provides a new method and means for objectively evaluating the origin, authenticity, merits, etc. of Chinese herbal medicines.