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作者将来自日本的水痘病毒Oka株,在MRC-5人成纤维细胞上繁殖,收获感染细胞,浓缩,用稳定剂稀释,然后破坏细胞使之释放胞内的水痘-带状疱疹病毒(VZV),最后冻干,制成两批原始高滴度疫苗。将这两批疫苗于37℃放置10天,模拟长期贮存的老化过程,得到相应低滴度疫苗(热处理疫苗),安慰剂除无VZV外,制备方法与原始活疫苗相同。
The authors breed the varicella-virus Oka strain from Japan on MRC-5 human fibroblasts, harvest the infected cells, concentrate, dilute with stabilizers, and then destroy the cells to release intracellular varicella-zoster virus (VZV) , And finally lyophilized to make two batches of the original high-titer vaccine. The two batches of vaccine were placed at 37 ° C for 10 days to simulate the long-term storage aging process to obtain the corresponding low-titer vaccine (heat-treated vaccine). The placebo was prepared in the same manner as the original live vaccine except for the absence of VZV.