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产量性状是玉米育种的首要目标,但产量相关基因的精细定位与克隆进展缓慢.OsAPO1是水稻中一个控制花序结构的基因,它具有多种功能,既能提高抗倒伏能力又能影响作物的产量.本研究在玉米中克隆了OsAPO1的同源序列ZmAPO1-6和ZmAPO1-9.蛋白多序列比对发现,2个玉米序列和OsAPO1相似性均超过了83%而且都含有F-box结构域.组织特异性表达分析表明,ZmAPO1主要在孕穗期的雌穗中表达.利用近200个自交系与S型细胞质雄性不育系测交构建了关联分析群体,在2个环境下对这2个基因进行了关联分析.在ZmAPO1-6和ZmAPO1-9上分别检测到14和8个与产量相关性状显著关联的位点,其中前者检测到的显著位点主要与株高和茎粗有关,而后者检测到的位点主要与百粒重有关.初步的研究结果推测,OsAPO1在玉米中的2个同源基因可能与产量和产量相关性状有关,该同源基因的克隆和初步分析可为进一步功能研究提供参考.
Yield traits are the primary target of maize breeding, but the fine mapping and cloning of yield-related genes is slow.OsAPO1 is a gene controlling inflorescence structure in rice, which has many functions, which can not only improve lodging resistance but also affect crop yield In this study, the homologous sequences of OsAPO1, ZmAPO1-6 and ZmAPO1-9, were cloned from maize and found that the similarity of the two maize sequences and OsAPO1 were more than 83% and both contained the F-box domain. Tissue-specific expression analysis showed that ZmAPO1 was mainly expressed in the ear of booting stage, and the correlation analysis population was established by crossing with nearly 200 inbred lines and S-type cytoplasmic male sterile line, and the two groups The results showed that 14 and 8 loci related to yield-related traits were detected in ZmAPO1-6 and ZmAPO1-9, respectively, of which the significant sites detected in the former were mainly related to plant height and stem diameter The results showed that two homologous genes of OsAPO1 in maize may be related to the traits related to yield and yield. Cloning and preliminary analysis of this homologous gene may be based on one Function of reference.