EB病毒早期抗原(EA)的抗体(EA—IgA和EA—IgG)测定,对鼻咽癌具有诊断和预后意义。为了建立EA抗体检测方法,特别是ELISA法,需激活EB病毒,诱导携带EB病毒基因组的非增殖性细胞株(Raji细胞)EA抗原的大量合成,增加其EA阳性细胞数。许多化学物质如正丁酸钠,抗人Ig、BUDR、IUDR、PHA、TPA和血清因子,均能诱导EA合成。其中以TPA合并正丁酸钠,IUDR及血清因子效果最好。TPA The antibodies against EB virus early antigen (EA) (EA-IgA and EA-IgG) have diagnostic and prognostic significance for nasopharyngeal carcinoma. In order to establish an EA antibody detection method, in particular ELISA method, it is necessary to activate EB virus, induce large-scale synthesis of EA antigens of non-proliferating cell strains (Raji cells) carrying the EB virus genome, and increase the number of EA-positive cells. Many chemicals such as sodium butyrate, anti-human Ig, BUDR, IUDR, PHA, TPA, and serum factors all induce EA synthesis. Among them, TPA combined with sodium butyrate, IUDR and serum factor had the best effect. TPA