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我们利用自行安装的ＰＩＧ基因枪瞬时转化水稻悬浮细胞，对其操作参数及转化条件的优化进行了研究。ＰＩＧ基因枪结构简单，操作方便，价格及维持费很低。研究结果表明，在设计参数为轰击持续时间５０ｍｓ及样品室真空度为２９～３０ｉｎ．Ｈｇ时，其它操作参数的优化值为氦气压力６０ＰＳｌ，轰击高度为１７ｃｍ，缓冲网板高度为ｇｃｍ。水稻悬浮细胞在轰击前后分别进行高渗处理（含有０．５Ｍ甘露醇的ＬＳ０．５培养基）４ｈ和１０～１６ｈ可提高转化率４倍以上，在ＤＮＡ吸附过程中，用乙醇进行后处理比用水处理可提高转化率３．８倍。另外，用金粉为ＤＮＡ载体比钨粉效果好，转化量可提高一倍以上。这些结果证实ＰＩＧ基因枪的可靠性及稳定性。这对促进ＰＩＧ基因枪在植物基因工程中的应用有着重要意义。
We transiently transformed rice suspension cells by using the PIG gene gun we installed, and studied the optimization of its operating parameters and transformation conditions. PIG gene gun structure is simple, easy to operate, low price and maintenance costs. The results show that the design parameters for the bombardment duration of 50ms and the sample chamber vacuum 29 ~ 30in. Hg, the other operating parameters are optimized for a helium pressure of 60 PSI, a bombardment height of 17 cm, and a buffered screen height of gcm. Hypertonic treatment of rice suspension cells (LS0.5 medium containing 0.5M mannitol) for 4h and 10 ~ 16h before and after bombardment, respectively, improved the conversion rate by more than 4 times. During DNA adsorption, ethanol was used for post-treatment ratio Water treatment can improve the conversion rate of 3.8 times. In addition, the use of gold powder as a DNA carrier effect than tungsten powder, the conversion can be more than doubled. These results confirm the reliability and stability of the PIG gene gun. This is of great significance for the application of PIG gene gun in plant genetic engineering.