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目的:探讨胸苷磷酸化酶(TP)与口腔鳞癌(OSCC)临床病理及预后的关系,并研究其与微血管密度(microvessel density,MVD)的相关性。方法:用SP免疫组化法检测63例OSCC中TP的表达水平,利用CD34标记的血管进行MVD计数,对所有病例进行随访。采用SPSS13.0软件包进行统计学分析,以χ2检验分析TP的表达及其与各临床病理因素及MVD间的关系,应用寿命表法完成对病例随访资料的生存状况评价。结果:TP在口腔鳞癌组织中的表达显著高于正常口腔黏膜组织(P<0.01);TP的表达与肿瘤的颈淋巴结转移密切相关(P<0.01),而与患者性别、年龄、临床分期及病理分化程度无明显相关(P>0.05);TP表达阳性组的MVD高于TP表达阴性组(P<0.05);TP的表达与MVD呈正相关(P<0.05)。生存分析显示,TP阴性组的5年生存率高于TP阳性组,术后复发组的TP表达阳性率(90.9%)显著高于术后无复发者(50.0%)(P<0.05)。结论:TP的表达水平与口腔鳞癌的颈淋巴结转移、血管新生及预后密切相关,可以作为其一项不良因子。
Objective: To investigate the relationship between thymidine phosphorylase (TP) and the clinicopathology and prognosis of oral squamous cell carcinoma (OSCC) and to investigate its relationship with microvessel density (MVD). Methods: The expression of TP in 63 cases of OSCC was detected by SP immunohistochemical method. MVD was counted by CD34 labeled vessels and all the cases were followed up. SPSS13.0 software package was used for statistical analysis, χ2 test was used to analyze the expression of TP and its relationship with clinicopathological factors and MVD, and to evaluate the survival status of case follow-up data by life table method. Results: The expression of TP in oral squamous cell carcinoma was significantly higher than that in normal oral mucosa (P <0.01). The expression of TP was closely related to the cervical lymph node metastasis (P <0.01), but not to the gender, age, clinical stage (P> 0.05). The MVD of TP positive group was higher than that of TP negative group (P <0.05). The expression of TP was positively correlated with MVD (P <0.05). Survival analysis showed that the 5-year survival rate of TP-negative group was higher than that of TP-positive group. The positive rate of TP expression (90.9%) in postoperative recurrence group was significantly higher than that of non-recurrence group (50.0%) (P <0.05). Conclusion: The expression level of TP is closely related to cervical lymph node metastasis, angiogenesis and prognosis in oral squamous cell carcinoma, which may serve as an adverse factor.