论文部分内容阅读
目的观察ＩＬ－４基因转染对ＨＬ－６０增殖及Ｔ细胞功能的影响及其可能机制。方法采用逆转录病毒载体介导基因转染法将人ＩＬ－４基因导入髓系白血病细胞ＨＬ－６０，观察高表达外源性ＩＬ－４基因ＨＬ－６０株的增殖反应及其诱导的正常人ＰＢＭＣ增殖，ＩＬ－２、ＩＬ－６、ＩＦＮ－γ基因表达及肿瘤特异性ＣＴＬ杀伤活性。结果ｒＩＬ－４或瘤细胞产生的ＩＬ－４早期促进ＨＬ－６０细胞增殖，培养５天后则明显抑制其增殖；与ｒＩＬ－４处理的ＨＬ－６０细胞比较，ＩＬ－４基因修饰的ＨＬ－６０细胞明显促进正常人ＰＢＭＣ增殖并合成ＩＬ－２、ＩＬ－６及ＩＦＮ－γｍＲＮＡ，其诱导的特异性ＣＴＬ杀伤活性明显高于野生型ＨＬ－６０、仅导入载体的ＨＬ－６０和加入ｒＩＬ－４共育的ＨＬ－６０细胞诱导者。结论ＩＬ－４基因转染在影响ＨＬ－６０细胞增殖和分化过程的同时，可能促进某些抗原（如ＭＨＣⅠ类抗原）的抗原性，从而有利于机体建立有效的抗肿瘤免疫反应。
Objective To investigate the effect of IL-4 gene transfection on the proliferation and T cell function of HL-60 and its possible mechanism. Methods Human IL-4 gene was transfected into HL-60 myeloid leukemia cells by retroviral vector-mediated gene transfection method. The proliferative response of HL-60 gene and the normal human induced by it PBMC proliferation, IL-2, IL-6, IFN-γ gene expression and tumor-specific CTL cytotoxicity. Results The IL-4 produced by rIL-4 or tumor cells promoted the proliferation of HL-60 cells at early stage and inhibited the proliferation of HL-60 cells after 5 days of culture. Compared with HL-60 cells treated with rIL-4, IL-4 gene modified HL- The proliferation of normal human PBMCs was significantly enhanced and the levels of IL-2, IL-6 and IFN-γ mRNA were significantly increased in PBMCs. The specific cytotoxic activity against CTLs was significantly higher than that of wild type HL-60 cells and only in HL-60 and rIL-4 Co-incubation of HL-60 cell inducer. CONCLUSION: IL-4 gene transfection may affect the antigenicity of some antigens (such as MHC class I antigens) while affecting the proliferation and differentiation of HL-60 cells, which will help the body to establish an effective anti-tumor immune response.