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目的获得适宜悬浮培养的刺五加愈伤组织。方法利用植物组织和细胞培养技术,考察3种诱导培养基及9种继代培养基对刺五加愈伤组织的诱导及愈伤组织状态的影响。结果以α-萘乙酸(α-Naphthaleneacetic acid)2.0 mg.L-1+6-苄氨基嘌呤[N-(phenylmethyl)-1H-purin-6-amine,6-BA]2.0 mg.L-1诱导出的愈伤组织适于悬浮培养,叶和叶柄的诱导率分别为93.3%和100%;筛选出最佳固体继代培养的外源激素配比为NAA1.0 mg.L-1+6-BA1.0 mg.L-1,在此激素配比下,愈伤组织生长代谢迅速,性状稳定,呈易分散的颗粒状,易于分散,适合下一步的悬浮培养。结论以刺五加嫩叶为外植体,NAA2.0 mg.L-1+6-BA2.0 mg.L-1为诱导培养基,NAA1.0 mg.L-1+6-BA1.0 mg.L-1为继代培养基可以获得适宜悬浮培养的愈伤组织。
Objective To obtain Acanthopanax senticosus callus suitable for suspension culture. Methods Plant tissue and cell culture techniques were used to investigate the effects of three kinds of induction medium and nine kinds of subculture medium on callus induction and callus status of Acanthopanax senticosus. The results were induced with α-Naphthaleneacetic acid 2.0 mg.L-1 + 6-BA (N- (phenylmethyl) -1H-purin-6-amine, 6-BA] The callus was suitable for suspension culture. The induction rates of leaf and petiole were 93.3% and 100%, respectively. The optimum ratio of exogenous hormones of NAA1.0 mg.L-1 + 6- BA1.0 mg.L-1. Under the hormone ratio, the callus grew quickly and metabolized rapidly, with stable characters. It was easy to disperse granularity and easy to disperse, suitable for the next step of suspension culture. Conclusion NAA 2.0 mg.L-1 + 6-BA 2.0 mg.L-1 was used as explants and NAA 1.0 mg.L-1 + 6-BA1.0 mg.L-1 for subculture medium can be obtained suitable suspension culture callus.