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目的测定人乳铁蛋白转基因羊奶及非转基因羊奶中VB1、VB2及尼克酰胺的含量,考察其在2种羊奶中是否存在显著差异。方法 HPLC法测定泌乳30 d、60 d及90 d采集的2种羊奶中各90个样品的上述3种维生素含量,并进行统计学分析。结果泌乳30 d、60 d及90 d采集转基因羊奶,VB1含量分别为0.151μg/ml、0.102μg/ml、0.068μg/ml,VB2含量分别为1.622μg/ml、0.784μg/ml、1.228μg/ml,尼克酰胺含量分别为23.28μg/ml、8.032μg/ml、5.346μg/ml。结论 60 d及90 d,转基因羊奶与非转基因羊奶的VB1含量存在显著差异,30 d及60 d,2种羊奶的VB2含量存在显著差异。在3个时间段采集的样品中,2种羊奶的尼克酰胺含量均差异具有统计学意义(P≤0.05)。
OBJECTIVE To determine the contents of VB1, VB2 and nicotinamide in human lactoferrin and non-transgenic goat’s milk, and to investigate whether there is a significant difference between the two kinds of goat’s milk. Methods The HPLC method was used to determine the three vitamins in 90 samples of 2 kinds of goat milk collected on the 30th, 60th and 90th days of lactation, and the statistical analysis was made. Results The results showed that the VB1 contents of transgenic goat milk were 0.151μg / ml, 0.102μg / ml and 0.068μg / ml, VB2 contents were 1.622μg / ml, 0.784μg / ml and 1.228μg / ml, and the nicotinamide contents were 23.28 μg / ml, 8.032 μg / ml and 5.346 μg / ml, respectively. Conclusions At 60 d and 90 d, VB1 contents of transgenic goat milk and non-transgenic goat milk were significantly different. On the 30th and 60th day, there were significant differences in VB2 content between the two goat’s milk. There were significant differences in the contents of nicotinamide between the two kinds of goat milk samples collected in three time periods (P≤0.05).