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本文采用 PCR微板核酸杂交结合 ELISA检测显示技术对 74例乙型肝炎病毒 ( HBV)慢性感染者血清标本进行检测 ,研究 HBV C基因启动子 ( BCP)1 762 A→T、1 764G→ A双突变 ,探讨 BCP变异对HBe系统的影响及其意义。1 对象与方法1 .1 对象 :74例患者均为 1 999年 1 2月～ 2 0 0 1
In this paper, 74 cases of chronic hepatitis B virus (HBV) serum samples were detected by PCR microplate nucleic acid hybridization combined with ELISA detection serum HBV C gene promoter (BCP) 1 762 A → T, 1 764G → A double Mutation, to explore the impact of BCP mutation on HBe system and its significance. 1 Subjects and methods 1.1 Object: 74 patients were January 999 1 ~ 2 0 0 1