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1987年3月以“化杀”杂种（IR_（24）×紫圭、IR_（661）×紫圭、135×紫圭）F_1代种子和其4个亲本种子（含水量13％）为供试材料,用279Gy r-射线（f=0.93）进行辐照处理,以相应的未辐照种子作对照,分别播种、移栽,到幼穗分化期时消毒后在无菌条件下剥取幼穗,制成0.2～0.3厘米大小的外植体,分别接种到诱导培养基上进行暗培养,8～10天后各组均有部分外植体发生颗粒状、淡黄色的愈伤组织,到30天左右,愈伤组织多数是0.1～0.3厘米大的颗粒,结构致密,有部分聚集成0.3～0.5厘米大的块状,结构疏松,易散开成颗粒。30天时移至分化培养基中在26～28℃、光照9～10小时（光强2000勒克斯）条件下进行分化培养,4～5天后部份愈伤组织有的发生绿点,有的产生白色茸毛状新愈伤组织突起;8～10天后出现绿芽和根的分化,分化的绿芽有丛生。每块愈伤组织可分化出10个左右绿芽,少的仅
In March 1987, F1 hybrids (IR_ (24) × Ziwei, IR_ (661) × Ziwei, 135 × Ziwei) F1 seeds and their 4 parents seeds (water content 13%) were used as the test Materials, with 279Gy r-ray (f = 0.93) for irradiation, with the corresponding non-irradiated seeds as a control, respectively, sowing, transplanting, to young panicle during the period after disinfection under sterile conditions stripped ears , 0.2-0.3 cm explants were made and inoculated into induction medium for dark culture respectively. After 8 to 10 days, some explants in each group had granular and light yellow callus, and after 30 days , The majority of callus is 0.1 ~ 0.3 cm large particles, compact structure, some aggregated into 0.3 ~ 0.5 cm large block, loose structure, easy to spread into granules. 30 days to differentiation medium at 26 ~ 28 ℃, light 9 to 10 hours (light intensity 2000 lux) under the conditions of differentiation and culture, 4 to 5 days some of the callus some green spots occur, and some produce white Hairy new callus protrusions; 8 to 10 days after the emergence of green shoots and roots differentiation, differentiation of green shoots have clusters. Each callus can differentiate about 10 green shoots, less only