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为了分析肠道内主要草酸降解细菌与肾结石疾病的相关性,本研究采集 29 名健康对照和 30 名肾结石患者的粪便样品提取微生物基因组 DNA,采用实时荧光定量 PCR 的方法,检测产甲酸草酸杆菌Oxalobacter formigenes)、乳杆菌属细菌 (Lactobacillus)、双歧杆菌属细菌 (Bifidobacterium) 和肠杆菌科细菌(Enterobacteriaceae)这 4 种草酸降解细菌功能类群在总菌中相对含量的变化。结果显示 O. formigenes、Lact-%obacillus 和 Bifidobacterium 在肾结石病例组与健康对照组的肠道内的丰度无显著差异,而 Enterobacteriaceae在两组之间的差异显著。与健康对照组相比,Enterobacteriaceae 在肾结石病例组的丰度偏高(p=0.02);将肾结石病例组按照结石发生位置分为单发组和多发组,Enterobacteriaceae 在健康对照组与结石单发组的丰度无显著差异,但在结石多发组的丰度都显著高于健康对照组(p=0.001)与结石单发组(p=0.042)。结果提示Enterobacteriaceae 丰度可以作为一个灵敏的生物学指标,用于预测和评价宿主罹患多发肾结石的风险,有助于更深入的研究宿主和肠道菌群之间在肾结石发生发展中的相互作用,为从维持肠道微生态平衡角度进行针对性治疗提供参考。
In order to analyze the correlation between the major oxalate-degrading bacteria in the intestine and the diseases of nephrolithiasis, we collected genomic DNA from 29 healthy controls and 30 stool samples from patients with kidney stones. Real-time PCR was used to detect the presence of oxalic-acid- Oxalobacter formigenes, Lactobacillus, Bifidobacterium and Enterobacteriaceae in the total bacteria. The results showed that O. formigenes, Lact-% obacillus and Bifidobacterium had no significant difference in the intestinal tract abundance between the kidney stone group and the healthy control group, while Enterobacteriaceae had significant difference between the two groups. Compared with the healthy control group, Enterobacteriaceae was more abundant in the nephrolithiasis group (p = 0.02). The nephrolithiasis group was divided into single-episode group and multiple-episode group according to the location of stones. Enterobacteriaceae was significantly higher in the control group There was no significant difference in abundance in the hair group, but the abundance in the multiple stone group was significantly higher than that in the healthy control group (p = 0.001) and stones alone group (p = 0.042). The results suggest that the abundance of Enterobacteriaceae can be used as a sensitive biological indicator to predict and evaluate the host’s risk of developing multiple kidney stones and to further investigate the relationship between host and intestinal flora in the development of kidney stones Provide a reference for the targeted treatment to maintain the balance of intestinal microflora.