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非洲紫罗兰是著名的温室盆栽花卉,品种多,花色杂。用叶插繁殖,需时长、速度慢,且一片叶子只能繁殖几株。1987年来我们用组织培养法进行繁殖,初见成效。方法如下:将非洲紫罗兰的叶片,切成0.5—0.8平方厘米的小片,作为外植体,接种到 MS 附加其它成分的培养基上进行培育。离体叶片在培育基上培养2周后,叶片由绿变黄,逐渐增大变厚,形成愈伤组织,其表面逐渐出现密集的小突起,初为黄白色,后变绿色,进而分化成小芽。
African violets are famous greenhouse potted flowers, variety, color miscellaneous. Reproduction with leaves, take time, slow, and only a few strains of a leaf. In 1987, we used tissue culture method to reproduce, and achieved initial success. The method is as follows: The leaves of African violets are cut into small pieces of 0.5-0.8 cm 2, and the explants are inoculated into a medium supplemented with other components of MS to be cultured. After cultured in vitro for two weeks, the leaves changed from green to yellow, gradually increased and thickened, forming callus. The surface of the leaves was densely populated with small protuberances. The initial yellowish-white color turned green and then differentiated into Small buds.