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本文报道在植物组织培养中,~(60)Co_(-γ)射线对培养基的消毒作用,旨在探明,用~(60)Co_(-γ)射线消毒培养基的可行性及对培养效果的影响。结果表明,经~(60)Co_(-γ)射线600krad 处理培养基能达到灭菌。其培养基能促进愈伤组织的生长,而对绿苗分化率没有影响。辐射导致的茶色培养瓶可促进水稻愈伤组织根的分化和生长而抑制芽的分化。
This paper reports the disinfection effect of ~ (60) Co _ (- γ) radiation on the culture medium in plant tissue culture in order to find out the feasibility of using 60Co_ (- γ) Effect of effect. The results showed that the treatment of 600krad by ~ (60) Co _ (- γ) radiation could achieve sterilization. The medium can promote the growth of callus, but has no effect on the rate of green plantlet differentiation. Radiation-induced brown flask can promote the differentiation and growth of rice callus roots and inhibit bud differentiation.