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利用共焦荧光显微镜在单个分子水平上检测了罗丹明标记的磷脂分子(T1 391 )在溶液中和玻璃表面的扩散行为 ,并获得了单个分子分辨的荧光成像 .用穿越时间分布函数拟合分子通过焦点时发射荧光信号的宽度分布 ,得到 T1 391分子在水溶液中的扩散常数为 2 .3× 1 0 - 6 cm2 ·s- 1, 在亲水玻璃表面上的扩散常数为 3.4× 1 0 - 8cm2 ·s- 1.部分磷脂分子与玻璃表面发生强吸附作用 ,其停留在焦点内的时间较长 ,特征穿越时间为 85ms
The diffusion behavior of rhodamine-labeled phospholipid molecule (T1 391) in solution and on the glass surface was detected by confocal fluorescence microscopy at a single molecular level and a single molecularly resolved fluorescence image was obtained. The molecule was fitted by crossing time distribution function The distribution of the fluorescence signal width at the time of focal distribution shows that the diffusion constant of T1 391 molecule in aqueous solution is 2.3 × 10 -6 cm2 · s -1 and the diffusion constant on the hydrophilic glass surface is 3.4 × 10 4 - 8cm2 · s- 1. Part of the phospholipid molecules and the glass surface of the strong adsorption, the stay in focus for a long time, the characteristic traversal time of 85ms