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目的:探讨不同浓度的钼营养水平对甘草生长和生理特性的影响。方法:以一年生的甘草移栽苗为试验材料,采用盆栽蛭石的试验方法,共设置4个钼浓度水平,分别为0mg·L-1,0.52mg·L-1,5.2mg·L-1和10.4mg·L-1,其中0.52mg·L-1即正常Hoagland营养液中钼的浓度。每周向盆内浇灌营养液,以达到处理的目的。采用LI-6400光合仪测定其光合生理指标以及植物生理学常规方法进行甘草叶片色素和抗氧化酶活性的测定。采用电子天平分别测定不同处理下的甘草地上、根的鲜重和干重等。结果:结果表明,甘草的各项生理和生长指标随着钼处理浓度的增加而增加,显著增加了甘草植株的叶绿素a,总叶绿素,类胡萝卜素和净光合速率(Pn)、胞间CO2浓度(Ci)等光合指标以及显著提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,统计分析结果表明,各处理间差异显著(P<0.05)。同样,钼营养显著增加了甘草株高、芦头直径、生物量等生长指标。其中,10.4mg·L-1钼处理时的甘草根鲜重和干重最大,与0 mg·L-1处理相比,分别显著增加了55.35%和38.08%。结论:钼不足会抑制甘草的各项生理功能,进而影响甘草的生长,而5.2mg·L-1和10.4mg·L-1的钼营养浓度可以促进一年生甘草各项生理和生长指标的增加,进而促进甘草干物质的积累,提高甘草药材的产量。
Objective: To investigate the effects of different concentrations of molybdenum nutrition on the growth and physiological characteristics of Glycyrrhiza uralensis. Methods: The annual licorice transplanted seedlings as test materials, the use of potted vermiculite test methods, set a total of four levels of molybdenum, were 0mg · L-1, 0.52mg · L-1, 52mg · L-1 And 10.4 mg · L-1, of which 0.52 mg · L-1 was the concentration of molybdenum in the normal Hoagland nutrient solution. Weekly to the basin watering nutrient solution, in order to achieve the purpose of treatment. LI-6400 photosynthetic apparatus was used to determine the photosynthetic physiological parameters and plant physiology methods for the determination of leaf pigment and antioxidant enzyme activity. The electronic balance was used to determine the fresh weight and dry weight of licorice under different treatments. Results: The results showed that the physiological and growth indexes of Glycyrrhiza uralensis increased with the increase of molybdenum concentration, significantly increased the contents of chlorophyll a, total chlorophyll, carotenoid and Pn, (Ci) and other photosynthetic indexes as well as the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly increased. Statistical analysis showed that the differences among treatments were significant (P <0.05) . Similarly, molybdenum nutrition significantly increased the growth of licorice plant height, root diameter, biomass and other growth indicators. Among them, fresh weight and dry weight of licorice roots were the highest when treated with 10.4 mg · L-1 molybdenum, which increased 55.35% and 38.08% respectively compared with 0 mg · L-1 treatment. Conclusion: Molybdenum deficiency can inhibit the physiological functions of licorice and further affect the growth of Glycyrrhiza uralensis. The molybdenum concentrations of 5.2 mg · L-1 and 10.4 mg · L-1 can promote the increase of physiological and growth indexes of annual licorice, Thereby promoting the accumulation of licorice dry matter and increasing the yield of licorice medicinal materials.