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目的 :探查孕妇外周血清胎盘生长因子(PLGF)、抑制素A(INH-A)和人绒毛膜促性腺激素(β-HCG)在正常妊娠及子痫前期患者中的表达差异,探讨其在子痫前期发病中的作用及临床应用价值。方法 :用ELISA法和CLIA法分别检测46例子痫前期孕妇(其中轻度子痫前期孕妇24例、重度子痫前期孕妇22例)及28例正常孕妇外周血清标本中PLGF、INH-A、β-HCG的浓度,比较其在正常妊娠组和不同病情程度子痫前期组中的水平差异;应用ROC曲线和logistic回归评价各指标单独及联合运用对子痫前期的诊断准确性。结果 :孕妇血清PLGF水平正常妊娠组>轻度子痫前期组>重度子痫前期组,INH-A、β-HCG水平正常妊娠组<轻度子痫前期组<重度子痫前期组,各组间差异均具有统计学意义(p<0.05)。各指标诊断子痫前期的ROC曲线下面积分别为:(AUC)PLGF=0.811、(AUC)INH-A=0.844、(AUC)β-HCG=0.768,指标联合后ROC曲线下面积增大。结论 :PLGF、INH-A、β-HCG可能参与了子痫前期的发生发展,其水平与子痫前期病情严重程度有一定相关性,可作为子痫前期诊断及病情评估的辅助指标,联合运用准确性更高。
Objective: To investigate the difference of expression of placental growth factor (PLGF), inhibin A (INH-A) and human chorionic gonadotropin (β-HCG) in normal pregnant women and preeclampsia pregnant women, The role of preeclampsia and its clinical value. Methods: The expressions of PLGF, INH-A and β in 46 cases of preeclampsia (24 cases of mild preeclampsia and 22 cases of severe preeclampsia) and 28 cases of normal pregnant women were detected by ELISA and CLIA respectively. -HCG concentrations were compared between the normal pregnancy group and different severity of preeclampsia group level differences; the use of ROC curve and logistic regression to evaluate the indicators of individual and combined use of the diagnosis of preeclampsia accuracy. Results: Serum levels of PLGF in pregnant women were significantly higher than those in normal pregnancy group, mild preeclampsia group, severe preeclampsia group, INH-A and β-HCG levels in normal pregnancy group, mild preeclampsia group, severe preeclampsia group, The difference between the two groups was statistically significant (p <0.05). The area under the ROC curve of each index in diagnosing preeclampsia were: (AUC) PLGF = 0.811, (AUC) INH-A = 0.844, (AUC) β-HCG = 0.768. CONCLUSION: PLGF, INH-A and β-HCG may be involved in the development of preeclampsia. The levels of PLGF, INH-A and β-HCG may be related to the severity of preeclampsia and may be used as auxiliary indicators for the diagnosis and assessment of preeclampsia. More accurate.