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该试验以腋芽和胚为外植体,进行了梅花愈伤组织的长期培养及其细胞学的初步观测.结果表明成年母株腋芽外植体启动培养的愈伤率和发芽率在不同品种和不同培养基之间稍有差异,3#梅花品种和MS+NAA2.0+BA1.0+ZT2.0mg·L-1培养基较高.胚在MS+GA10.0培养基上发芽率高,并形成正常无根苗.暗培养有利于愈伤组织的生长,光培养有利于愈伤组织的分化.水解酪蛋白(CH)既有利于愈伤组织的生长、增殖,也延长了愈伤组织的寿命.梅花愈伤组织在第四代达到生长高峰,附加CH之后在第七代又出现一次生长高峰;一代之内第四周生长最快.多次继代培养的结果还表明,MS+NAA5.0+KT1.0+CH1000有利于梅花愈伤组织的长期培养.细胞学观测的结果表明,多次继代培养的梅花愈伤组织的细胞形状不规则,核物质相对稀少,并且分化出了很多导管.
In this experiment, axillary buds and embryos were used as explants for long-term culture of plum flower callus and preliminary observations of their cytology. The results showed that the callus rate and germination rate of axillary bud explants of adult female parental plants were slightly different between different cultivars and different mediums. Compared with MS + NAA2.0 + BA1.0 + ZT2.0mg · L-1 medium high. Embryos in MS + GA10.0 medium germination rate is high, and the formation of normal rootless seedlings. Dark culture is conducive to the growth of callus, light culture is conducive to the differentiation of callus. Hydrolysis casein (CH) both conducive to callus growth, proliferation, but also extend the callus life. The plum blossom callus reached the peak of growth in the fourth generation, and another peak appeared in the seventh generation after the addition of CH. The fastest growth occurred in the fourth week. The results of multiple subcultures also showed that MS + NAA5.0 + KT1.0 + CH1000 was beneficial to long-term culture of plum callus. Cytological observations showed that the plumule callus of multiple subcultures had irregularly shaped cells with relatively few nuclear materials and many differentiated catheters.