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目的探讨番茄红素对脂多糖(LPS)所诱导的RAW264.7巨噬细胞炎症反应的影响。方法采用Griess法和酶联免疫吸附测定(ELISA)法检测一氧化氮(NO)和白介素-6(IL-6)的生成水平,半定量逆转录-聚合酶链反应(RT-PCR)和免疫印迹(Western-blot)法检测诱导型一氧化氮合酶(iNOS)和IL-6的基因表达量及iNOS的蛋白表达量。结果番茄红素中、高剂量组NO分别生成(40.6±1.5)、(26.6±1.3)μmol/L,均明显低于阳性对照组的(56.3±2.5)μmol/L(F=18.3,P<0.01);番茄红素低、中、高剂量组IL-6分泌量分别为(508.3±39.5)、(469.2±66.9)、(335.5±72.9)pg/mL,均明显低于阳性对照组的(603.4±16.8)pg/mL(F=92.6,P<0.05或P<0.01),且其iNOS和IL-6基因及蛋白表达水平也均明显低于阳性对照组。结论番茄红素能够降低LPS所诱导的RAW264.7巨噬细胞中iNOS、IL-6及其产物NO、IL-6的表达水平,提示其可能对于治疗各种炎症相关性疾病如动脉粥样硬化等有着重要意义。
Objective To investigate the effect of lycopene on the inflammatory response induced by lipopolysaccharide (LPS) in RAW264.7 macrophages. Methods Griess method and enzyme linked immunosorbent assay (ELISA) were used to detect the levels of nitric oxide (NO) and interleukin-6 (IL-6), semiquantitative RT-PCR The gene expression of iNOS and iNOS and the protein expression of iNOS were detected by Western-blot. Results Compared with the positive control group, the levels of NO in the medium and high doses of lycopene were (40.6 ± 1.5) and (26.6 ± 1.3) μmol / L, respectively, which were significantly lower than those in the positive control group (56.3 ± 2.5μmol / L, F = 0.01). The levels of IL-6 in lycopene group were (508.3 ± 39.5), (469.2 ± 66.9) and (335.5 ± 72.9) pg / mL respectively, which were significantly lower than those in the positive control group 603.4 ± 16.8) pg / mL (F = 92.6, P <0.05 or P <0.01), and the expression of iNOS and IL-6 gene and protein were also significantly lower than that of the positive control group. Conclusions Lycopene can reduce the expression of iNOS, IL-6 and NO, IL-6 in LPS-induced RAW264.7 macrophages, suggesting that it may play an important role in the treatment of various inflammatory-related diseases such as atherosclerosis It is of great significance.