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为明确小麦体细胞无性系4-8(WS4-8)抗条锈病的遗传稳定性及抗性遗传特点,采用基因推导、抗性鉴定、遗传分析等方法对其进行了抗条锈性的鉴定和等位性分析。结果表明,WS4-8所携带的抗性基因与已知抗性基因不同;WS4-8的条锈病抗性表现优异,遗传稳定;用CY33小种对WS4-8和铭贤169的正交、反交组合F1和F2代植株人工接种鉴定表明,F1全部抗病,F2群体符合3R∶1S单基因控制的抗性遗传规律,WS4-8对CY33的抗性由1对显性核基因控制;用CY33对WS4-8分别与Yr5/6×Avocet S、Yr10/6×Avocet S、Yr15/6×Avocet S及92R137(Yr26)组配的杂交组合F1及F2代植株人工接种鉴定表明,F1全部抗病,而F2中有感病植株,说明WS4-8所携带的抗条锈病基因与Yr5、Yr10、Yr15、Yr26不等位。研究表明,WS4-8的抗条锈性是由1对显性核基因控制,与已知抗性基因不同,可能是一个新的抗条锈病基因。
In order to understand the genetic stability and genetic characteristics of resistance to stripe rust of wheat somaclonal 4-8 (WS4-8), the resistance to stripe rust was identified by genetic inference, resistance identification and genetic analysis And allelic analysis. The results showed that WS4-8 had different resistance genes and known resistance genes. Stripe rust resistance of WS4-8 was excellent and genetically stable. Artificially inoculated F1 and F2 offspring plants showed that F1 was resistant to all the disease and F2 population conformed to the genetic rule of 3R:1 single gene resistance. The resistance of WS4-8 to CY33 was controlled by one pair of dominant nuclear genes. The artificial inoculation of F1 and F2 plants with the combinations of CY33 and Yr5 / 6 × Avocet S, Yr10 / 6 × Avocet S, Yr15 / 6 × Avocet S and 92R137 (Yr26) Disease resistance, and susceptible plants in F2, indicating WS4-8 carried stripe rust resistance genes and Yr5, Yr10, Yr15, Yr26 notation. The results showed that the stripe rust resistance of WS4-8 was controlled by a pair of dominant nuclear genes, which might be a new stripe rust resistance gene with different known resistance genes.