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目的:研究确定痛风胶囊的定性定量方法。方法:采用薄层色谱法鉴别痛风胶囊中吴茱萸、陈皮和肉桂,采用高效液相色谱法测定痛风胶囊中吴茱萸碱含量。结果:薄层鉴别中,供试品色谱在与对照品和对照药材色谱相应位置上显相同颜色的斑点,阴性无干扰;含量测定中,吴茱萸碱在0.024～0.36μg范围内线性关系良好(r=1.000 0),平均加样回收率为98.60%(RSD为1.91%,n=6)。结论:薄层鉴别和含量测定方法操作简便、结果准确、重现性好,能有效控制痛风胶囊的质量。
Objective: To determine the qualitative and quantitative method of gout capsules. METHODS: TLC was used to identify Erigeron breviscapus, tangerine peel and cinnamon in Gout Capsule. The content of evodiamine in Gout Capsule was determined by high performance liquid chromatography. RESULTS: In the thin layer identification, the chromatogram of the test product showed the same color spots in the corresponding positions of the reference substance and the reference drug substance, and the negative was not interfered. In the content determination, the evodiamine had good linearity in the range of 0.024 to 0.36 μg (r =1.000 0). The average recovery was 98.60% (RSD was 1.91%, n=6). Conclusion: Thin layer identification and content determination methods are simple, accurate and reproducible, and can effectively control the quality of Gout Capsule.