Design of a multiplex PCR method for detection of toxigenicpathogenic in Vibrio cholerae

来源 :Asian Pacific Journal of Tropical Medicine | 被引量 : 0次 | 上传用户:pmlypmly
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Objective:To study virulence and regulatory genes(hlyA,ctxB,tcpI) in clinical strains of Vibrio ckolerae(V.cholerae),simultaneously.Methods:Three important genes,tepI,hlyA and ctxB were used for detection of toxigenic and pathogenic V.cholera by chain reaction assay method. Results:According to the results of the PCR,the incidence of hlyA,tcpI,and ctxB genes in clinical isolates was obtained as 94.7%(72 sample),90.8%(69 sample),and 92.1%(70 sample), respectively.Five strains possessed all genes except ctxB,six strains possessed all genes except tcpI,four strains possessed all genes except hlyA,one strain possessed only hlyA and 60 strains contained a combination of three genes.Including hlyA,ctxB and tcpI,Conclusions:Result show that this method could be reliable to detect toxigenic-pathogenic strains of V.cholerae in Iran. Objective: To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio ckolerae (V. cholerae), simultaneously. Methods: Three important genes, tepl, hlyA and ctxB were used for detection of toxigenic and pathogenic V. Cholera by chain reaction assay method. Results: According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Focal possessed all genes except ctxB, six-possessed all genes except tcpI, four-possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes. Including hlyA, ctxB and tcpI, Conclusions: Result show that this method could be reliable to detect toxigenic-pathogenic strain of V. cholerae in Iran.
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