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目的：ＣＤ４４基因是一种肿瘤转移相关基因。研究检测ＣＤ４４ｖ６蛋白在喉癌中的表达及其临床意义。方法：用ＣＤ４４ｖ６单克隆抗体，通过免疫组化Ｓ－Ｐ法，对６９例喉鳞状细胞癌蜡块标本和１０例正常喉粘膜标本进行研究，并结合临床资料进行预后分析。结果：６９例喉鳞状细胞癌、１０例正常喉粘膜中ＣＤ４４ｖ６蛋白阳性表达率分别为５２２％（３６／３９）和００％（０／１０）。ＣＤ４４ｖ６蛋白阳性表达与肿瘤发生部位、病理扮级和临床分期无相关性（Ｐ＞００５）；ＣＤ４４ｖ６蛋白阳性表达者颈淋巴结转移率（５０％，１８／３６）比阴性表达者高（１８２％，６／３３），两组有显著性差异（Ｐ＜００１）。用Ｌｏｇ＿ｒａｎｋ检验分析ＣＤ４４ｖ６蛋白表达阴性组生存率高于表达阳性组（Ｐ＜００５）。结论：ＣＤ４４蛋白的阳性表达与喉鳞癌颈淋巴结转移和生存率显著相关，ＣＤ４４ｖ６可能作为颈测喉癌转移和预后的一个重要的指标。
Objective: CD44 gene is a tumor metastasis-related genes. To investigate the expression of CD44v6 protein in laryngeal carcinoma and its clinical significance. Methods: The specimens of 69 specimens of laryngeal squamous cell carcinoma and 10 specimens of normal laryngeal mucosa were studied by CD44v6 monoclonal antibody by immunohistochemical S-P method. The prognosis was analyzed by clinical data. Results: The positive rates of CD44v6 protein expression in 69 cases of laryngeal squamous cell carcinoma and 10 cases of normal laryngeal mucosa were 522% (36/39) and 00% (0/10), respectively. The positive expression of CD44v6 protein had no correlation with the tumor location, pathology and clinical stage (P> 0.05). The positive rate of cervical lymph node metastasis (50%, 18/36) in CD44v6 positive group was higher than that in negative group 2%, 6/33), there was a significant difference between the two groups (P <0 01). Log_rank test analysis of CD44v6 protein expression negative group survival rate was higher than the positive expression group (P <0 05). Conclusion: The positive expression of CD44 protein is significantly correlated with cervical lymph node metastasis and survival rate of laryngeal squamous cell carcinoma. CD44v6 may be used as an important index for cervical metastasis and prognosis of cervical laryngeal carcinoma.