江西中医学院医学遗传研究室陶学训等同志,在省妇女保健院的密切配合下,经过3年艰苦努力,摸索出一种羊水细胞培养和染色体标本制备的简便方法。通过106份标本的培养,效果良好,成功率高。这种方法是抽取16～20周孕妇羊水20毫升,通过一定时间的培养后,即可获取新培养的羊水细胞。由于培养基的使用,纺缍体抑制剂的用量以及收获细胞的时机和方法等方面都作了重大的改进,所以能获得比较清晰的羊水细胞染色体。尤其是用自制竹质小棒直接从培养瓶内壁刮取细胞代替酶化学法这一 Jiangxi Medical College Medical Genetics Tao Xuexun and other comrades, in close cooperation with the Provincial Women’s Health Care Hospital, after three years of hard work, to explore a amniotic fluid cell culture and chromosome preparation of a simple method. Through the cultivation of 106 specimens, the effect is good and the success rate is high. This method is extracted from 16 to 20 weeks pregnant women, amniotic fluid 20 ml, after a certain period of training, you can get newly cultured amniotic fluid cells. Because of the use of medium, the amount of spinosad suppressant and the timing and method of harvesting cells have made significant improvements, so you can get a more clear amniotic cell chromosomes. Especially with homemade bamboo stick directly from the inner wall of the flask to scrape cells instead of enzyme chemistry