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为了探讨慢性铝暴露对大鼠铁代谢的影响,将100只4周龄清洁级雄性Wistar大鼠随机均分成摄铝组(430mg·L-1,以Al3+计)与对照组(蒸馏水),饮水染铝,每隔30d处死染铝大鼠和对照大鼠各10只,实验周期为150d.同时,在不同时间点(30d、60d、90d、120d、150d)测量大鼠体重,并进行协方差分析.最后,用火焰原子吸收分光光度法、固相竞争ELISA和比色法检测血浆Al、Fe、转铁蛋白(TF)、可溶性转铁蛋白受体(sTfR)含量及总铁结合力(TIBC).结果表明,在摄铝前期(90d前),染铝对大鼠体重无显著影响,而后期(120d后)染铝对大鼠体重抑制作用较大.在实验周期内,各时间点间对照组血浆中各指标均无显著差异,摄铝组随着时间的延长各指标变化较大.各时间点摄铝组血浆中Al含量及Al/Fe均显著高于对照组(p<0.01);30d时摄铝组血浆中Fe含量与对照组相比无显著差异(p>0.05),60、90、120d时均显著低于对照组(p<0.01),150d时显著高于对照组(p<0.01);30、60d时摄铝组血浆TF含量与对照组相比无显著差异(p>0.05),90d后显著高于对照组(p<0.05;p<0.01);30、60d时摄铝组TIBC与对照组相比无显著差异(p>0.05),其后各时间点均显著高于对照组(p<0.01);30d时摄铝组sTfR含量与对照组相比无显著差异(p>0.05),其后各时间点均显著低于对照组(p<0.01).由此可见,铝可干扰大鼠体内铁的代谢,影响铁的生物学作用.
In order to investigate the effect of chronic aluminum exposure on iron metabolism in rats, 100 clean Wistar rats of 4 weeks age were randomly divided into aluminum (430 mg · L-1, Al3 +) and control groups (distilled water) The rats were sacrificed and exposed to aluminum for 10 days every 10 days for 10 days.The experimental period was 150 days.At the same time, the body weight was measured at different time points (30d, 60d, 90d, 120d and 150d), and the covariance Finally, the contents of Al, Fe, TF, sTfR and TIBC in plasma were determined by flame atomic absorption spectrophotometry, solid-phase competition ELISA and colorimetric assay ) .The results showed that aluminum had no significant effect on the body weight of rats at the early stage of aluminum exposure (before 90 days), while the aluminum inhibited the body weight of rats at the late stage (after 120 days), and during the experimental period, There was no significant difference in the indexes of the plasma between the control group and the aluminum group with the prolongation of time.The contents of Al and Al / Fe in plasma were significantly higher than those in the control group at each time point (p <0.01) (P> 0.05). There was no significant difference between the two groups in the content of Fe (P> 0.05) at 60th, 90th and 120th days in the control group (P <0.01) (P <0.01). At 30 and 60 days, plasma TF levels in the aluminum group were not significantly different from those in the control group (p> 0.05), and were significantly higher than those in the control group after 90 days (p <0.05; p <0.01). At 30 and 60 days, there was no significant difference (P> 0.05) between the TIBC and the control group in the aluminum exposure group, and significantly higher than that in the control group at each time point (p <0.01) (P> 0.05), and then significantly lower than the control group at each time point (p <0.01) .Therefore, aluminum can interfere with iron metabolism in rats, affecting the biology of iron effect.