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美国衣阿华大学医学院Krieg等于1995年报道了一项惊人的发现,胞嘧啶和鸟苷(CPG)的简单两核苷酸序列是一种强烈的非特异性免疫刺激剂.在体外,CpGDNA显示激活B细胞,并能促使95%的B细胞进入细胞分裂期.CpG能使B细胞分泌白细胞介素(IL)6和免疫球蛋白.DNA基元亦能直接激活抗原提呈细胞.这种激活产生了一些能诱导与激活细胞毒T细胞有关的Thl免疫应答的细胞因子.Krieg为利用这项发现而建立的生物技术公司计划将CpG重复序列加入DNA疫苗以增强疫苗的免疫原性.此法亦可用于增强癌症蛋白疫苗的免疫原性.在最近发表的研究中,Krieg显示当分别用 CpG基元和弗氏完全佐剂作为抗38c13 B细胞淋巴瘤疫苗佐剂时,除CpG毒性较小外,两者的效力相同.
Krieg et al., Of the University of Iowa Medical School in the United States, reported a surprising discovery in 1995 that the simple two nucleotide sequence of cytosine and guanosine (CPG) is a strong nonspecific immunostimulant.In vitro, CpGDNA showed Activate B cells, and can promote 95% of B cells into the cell division.CpG enables B cells to secrete interleukin (IL) 6 and immunoglobulin.DNA elements also can directly activate antigen-presenting cells .This activation Several cytokines have been generated that induce a Thl immune response that is linked to the activation of cytotoxic T cells Biotechnology companies that Krieg has built to exploit this finding plan to add CpG repeats to DNA vaccines to enhance the immunogenicity of the vaccine Can also be used to enhance the immunogenicity of a cancer protein vaccine.In a recently published study, Krieg showed that when CpG motifs and Freund’s complete adjuvant were used as vaccine adjuvants against 38c13 B-cell lymphoma, respectively, except for small CpG toxicity In addition, the two have the same effect.