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目的为了提高毛乳头细胞培养的成功率和工作效率，并观察体外培养条件下毛乳头细胞的组织化学性质。材料与方法采用0．2％胶原酶D直接消化人产砂皮毛囊下部真皮鞘组织，分离出来的毛乳头予以悬浮培养，真皮鞘细胞和毛乳头细胞分别用DMEM培养基进行传代培训和多种组织化学染色。结果消化法显著降低了劳动强度，提高了培养成功率，加快了毛乳头细胞的生长，组织化学染色表明，毛乳头细胞阿新兰、甲苯胺O和PAS染色阳性，有异染性，龙是细胞凝集区和多层化细胞团中呈强阳性，而真皮勒细胞对阿新兰和甲苯胶蓝O染色呈阳性，无异染性，PAS阴性。结论消化法是一种简捷快速的高效培养毛乳头细胞的新方法，而毛乳头细胞是一类高度特殊化了的成纤维细胞，真皮鞘细胞与之关系紧密。
Objective To improve the success rate and working efficiency of dermal papilla cell culture and to observe the histochemical properties of dermal papilla cells under in vitro culture conditions. Materials and Methods Dermal sheath tissue of the lower part of human hair follicle was directly digested with 0.2% collagenase D. The isolated dermal papilla was suspended culture. Dermal sheath cells and dermal papilla cells were passaged and cultured in DMEM medium, Histochemical staining. Results Digestion method significantly reduced the labor intensity, increased the success rate of culture and accelerated the growth of dermal papilla cells. Histochemical staining showed that the dermal papilla cells were positive for Aspranin, Toluidine O and PAS, Cell agglutination zone and multi-layer cell mass was strongly positive, and Peyeri cells on the A New Zealand blue and toluene blue o staining was positive, no heterotropic, PAS negative. Conclusion Digestion is a simple and rapid method of culturing dermal papilla cells, a highly specialized type of fibroblast. Dermal sheath cells are closely related to it.