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测定血液或血浆中长春胺(Vincamine)已报道的方法有TLC、GC 和MS 法等。后者灵敏度最高,可以测出血浆药物浓度低至30 Pg/ml。作者用装有电位检测器的HPL C测定血浆中长春胺的浓度可低至5 ng/ml,标准曲线于5～500 ng/ml 范围内呈线性。将由HPLC 分离后的长春胺氧化产生的电流对施加电位(+0.8到1.3V)每步距增加50 mV 进样一次)制图,得到色谱电位曲线图。在Lichrosorb RP-8色谱柱上分离长春胺,用乙腈-水(1:1),0.05 M 过氯酸锂为洗脱剂,流速1.2 ml/min。
Vincamine in blood or plasma has been reported by TLC, GC and MS methods. The latter has the highest sensitivity and can measure plasma drug concentrations as low as 30 Pg / ml. The authors measured the plasma vincamine concentration as low as 5 ng / ml in HPL C with a potentiometric detector and a linear calibration curve in the range of 5 to 500 ng / ml. The current resulting from the oxidation of vinblastine after HPLC separation was plotted against an applied potential (+0.8 to 1.3 V) with an increase of 50 mV per step) to give a chromatogram potential plot. Vincamine was separated on a Lichrosorb RP-8 column using acetonitrile-water (1: 1) and 0.05 M lithium perchlorate as eluent at a flow rate of 1.2 ml / min.