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目的 :通过体外 MA和 TAM单药、不同序贯以及联合对乳腺癌 Bcap- 37细胞生长抑制作用研究 ,以指导临床选择合理的 TAM/ MA治疗方案。方法 :模仿临床而设计了 3个 TAM/ MA联合及两者单药的治疗方案 ,观察其体外对乳腺癌 Bcap- 37细胞的生长抑制作用 ,以 MTT法测定其细胞存活率。结果 :在低浓度时 TAM和MA体外对 Bcap- 37细胞的生长抑制作用相近 ,而 2 .5 mg/ L浓度以后 TAM的生长抑制作用显著高于 MA(P<0 .0 5 )。TAM→ MA序贯各浓度的肿瘤细胞生长抑制作用较 MA→ TAM序贯和 TAM+ MA联合为强 (P<0 .0 5 )。TAM+ MA和 MA→ TAM相比 ,在低浓度 (0 .2 5 m g/ L～ 2 .5 m g/ L)时两者肿瘤生长抑制作用相近。当 MA浓度为5 .0 m g/ L 和 7.5 m g/ L 时 ,联合用药组生长抑制作用更强 (分别为 P=0 .0 5和 P<0 .0 5 )。结论 :在 TAM与 MA不同联合治疗方案中以 TAM→ MA序贯或联合治疗方案为合理
Objective : To study the inhibition of breast cancer Bcap- 37 cells by MA and TAM in vitro, different sequential and combined to guide the selection of rational TAM/MA treatment. METHODS: Three TAM/MA combinations and two monotherapy regimens were designed to mimic the clinic and their growth inhibitory effects on breast cancer Bcap-37 cells were observed in vitro. Cell viability was measured by MTT assay. RESULTS: At low concentrations, the growth inhibitory effects of TAM and MA on Bcap-37 cells were similar in vitro, but the inhibitory effect of TAM on proliferation was significantly higher than MA at 2.5 mg/L (P<0.05). The sequential inhibition of tumor cells at different concentrations of TAM→ MA was stronger than MA→TAM sequential and TAM+ MA combined (P<0.05). Compared with TAM+ MA and MA→ TAM, tumor growth inhibition was similar at low concentrations (0.25 μg/L to 2.5 μg/L). When the MA concentration was 5.0 mg/L and 7.5 mg/L, the combination group showed stronger growth inhibition (P=0.05 and P0.05 respectively). Conclusion : It is reasonable to use TAM→ MA sequential or combination therapy in different combination therapy of TAM and MA