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目的探索骨髓基质细胞增强白血病细胞抗凋亡、抗药特性的可能机制。方法体外分离培养骨髓基质细胞模拟骨髓微环境功能,与白血病细胞Jurkat体外共培养。0.5μmol/L DNR处理Jurkat细胞诱导凋亡,应用AnnexinV/PI双标法流式细胞仪检测白血病细胞凋亡率。PI染色流式细胞仪检测细胞周期分布。结果0.1～2.0μmol/L浓度范围DNR作用一定时间后,Jurkat细胞发生的凋亡率随药物浓度的增加与作用时间的延长而升高。共培养后骨髓基质细胞抑制药物诱导的白血病细胞凋亡,白血病细胞凋亡率明显低于单独悬浮培养组(P<0.05)。白血病骨髓基质对白血病细胞的屏蔽效应强于正常骨髓基质细胞[白血病细胞凋亡率分别是(5.73±1.78)%,(8.39±4.08)%,(P<0.05)]。DNR处理共培养组Jurkat细胞G0/G1期阻滞,而正常与白血病骨髓基质细胞屏蔽的白血病细胞G0/G1期阻滞现象无显著性差异(P>0.05)。结论一定浓度的DNR在体外可诱导Jurkat细胞凋亡。骨髓基质细胞抑制化疗药物诱导的白血病细胞凋亡,这种保护作用可能部分通过阻滞白血病细胞于G0/G1期实现。白血病骨髓基质细胞对白血病细胞的屏蔽效应可能存在较细胞周期阻滞更复杂的机制。
Objective To explore the possible mechanism of bone marrow stromal cells enhancing anti-apoptosis and drug resistance of leukemia cells. Methods Bone marrow stromal cells were isolated and cultured to simulate the function of bone marrow microenvironment and co-cultured with leukemic cells Jurkat in vitro. Apoptosis was induced in Jurkat cells treated with 0.5μmol / L DNR. The apoptosis rate of leukemic cells was detected by Annexin V / PI double-labeled flow cytometry. PI staining flow cytometry to detect cell cycle distribution. Results The apoptosis rate of Jurkat cells increased with the increase of drug concentration and the prolongation of action time after DNR of 0.1 ~ 2.0μmol / L for a certain period of time. Coculture of bone marrow stromal cells inhibited drug-induced apoptosis of leukemia cells, apoptosis rate of leukemia cells was significantly lower than the suspension alone group (P <0.05). The leukemic bone marrow stromal cells had stronger shielding effect on leukemic cells than that of normal bone marrow stromal cells [(5.73 ± 1.78)%, (8.39 ± 4.08)%, P <0.05]. DNR treatment of Jurkat cells G0 / G1 phase arrest, while normal and leukemia bone marrow stromal cells leukemia cells G0 / G1 phase arrest no significant difference (P> 0.05). Conclusion A certain concentration of DNR can induce Jurkat cell apoptosis in vitro. Bone marrow stromal cells inhibit chemotherapeutic drug-induced apoptosis of leukemia cells, and this protective effect may be partially achieved by blocking leukemia cells in G0 / G1 phase. The screening of leukemic bone marrow stromal cells for leukemia cells may have a more complex mechanism than cell cycle arrest.