过去的许多研究已经证明,酶的活性中心是由酯解部位、疏水部位和阴离子部位组成,而活性中心周围还存在着两个以上的阴离子部位。不同结构的阳离子配体与酶结合可以使酶的性质发生明显的变化。目前研究最广泛的是一些双季铵或双吡啶盐类,它们大部分是酶的可逆性配体。它们有一些是酶的竞争性或部分竞争性抑制剂,有些则 Many past studies have shown that the active site of the enzyme is composed of an ester site, a hydrophobic site and an anionic site, and more than two anionic sites exist around the active site. Different structure of the cationic ligand and enzyme can make a significant change in the nature of the enzyme. Currently the most widely studied are some di-quaternary ammonium or bipyridyl salts, most of which are reversible ligands for enzymes. Some of them are competitive or partially competitive inhibitors of enzymes, while others are