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目的:观察人参皂甙Rd(ginsenoside Rd)对大鼠坐骨神经分支选择性损伤(spared sciatic nerve injury,SNI)引起的痛敏的影响及其作用机制。方法:坐骨神经分支选择性损伤术后7天,观察腹腔注射不同浓度人参皂甙Rd后大鼠后足的机械性缩足反应阈值(paw withdrawl mechanical threshold,PWMT)的变化;在术后7天,急性分离并取出大鼠腰4和腰5段背根节,对整节DRG上的中小型神经元运用全细胞膜片钳技术进行记录。结果:坐骨神经分支选择性损伤术后7天,大鼠出现明显的机械性痛敏,腹腔注射5 mg/ml和10 mg/ml的人参皂甙Rd能剂量依赖性的翻转大鼠机械性痛敏;坐骨神经分支选择性损伤能明显地增大SNI大鼠DRG中小型神经元上的钠电流以及减小电压依赖性钾电流,而100μM人参皂甙Rd能有效翻转该钠、钾电流的变化。结论:人参皂甙Rd能有效地改善坐骨神经分支选择性损伤引起的机械性痛敏,其机制可能与人参皂甙Rd明显地调节SNI大鼠DRG中小型神经元上的电压依赖性钠、钾电流有关。
Objective: To observe the effect of ginsenoside Rd on hyperalgesia induced by spared sciatic nerve injury (SNI) in rats and its mechanism. Methods: Selective sciatic nerve branches were sacrificed 7 days after the operation to observe the change of paw withdrawl mechanical threshold (PWMT) in rats’ hind paw after intraperitoneal injection of different concentrations of ginsenoside Rd. At 7 days after operation, acute The dorsal root ganglia of lumbar 4 and lumbar 5 were isolated and removed. Whole-cell patch-clamp technique was used to record the small and medium-sized neurons in the whole DRG. Results: Mechanical desensitization was observed in rats after selective injury of the sciatic nerve branches for 7 days. Ginsenoside Rd at 5 mg / ml and 10 mg / ml was able to reverse the mechanical hyperalgesia in a dose - dependent manner in rats. Selective damage of sciatic nerve branches can obviously increase sodium current and decrease voltage-dependent potassium current in DRG neurons of SNI rats, while 100μM ginsenoside Rd can effectively reverse the sodium and potassium currents. CONCLUSION: Ginsenoside Rd can effectively improve mechanical hyperalgesia induced by selective injury of sciatic nerve branches. The mechanism may be related to that Ginsenoside Rd significantly regulates voltage-dependent sodium and potassium currents in DRG neurons of SNI rats.