目的:研究中药九节茶对束缚应激小鼠免疫功能的改善作用。方法:实验将雄性C57BL/6小鼠分为正常对照组,应激对照组,125,500mg·kg-1九节茶提取物给药组,制备脾脏淋巴细胞悬液,流式细胞术分析T淋巴细胞亚群、NK细胞和NKT细胞比例及数目。结果:与应激对照组相比,中药九节茶可以提高束缚应激小鼠脾脏淋巴细胞总数,改善T淋巴细胞亚群比例,同时增加脾脏NK细胞和NKT细胞比例及数目。结论:中药九节茶可以改善应激负荷小鼠淋巴细胞数目及淋巴细胞亚群比例。 Objective: To study the effect of Jiuchica tea on immune function in restraint stress mice. Methods: Male C57BL / 6 mice were divided into normal control group, stress control group, 125,500mg · kg-1 Jiadu tea extract administration group to prepare spleen lymphocyte suspension, flow cytometry analysis of T lymphocytes Cell subsets, NK cells and NKT cell ratio and number. Results: Compared with the stress control group, Jiucha-te was able to increase the total number of spleen lymphocytes and the proportion of T lymphocyte subsets in the restraint stress-induced mice as well as the proportion and number of NK cells and NKT cells in the spleen. Conclusion: Chinese herbal medicine Jiutake can improve the stress-induced lymphocyte count and lymphocyte subsets in mice.